1.
To obtain haploid plant, we culture
A
Entire anther
B
Nucleus
C
Embryo
D
Apical bud
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To obtain haploid plant, we culture
Entire anther
Nucleus
Embryo
Apical bud
The production of secondary metabolites require the use of
Protoplast
Cell suspension
Meristem
Auxillary buds
The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is known as
Redifferentiation
Dedifferentiation
either a or b
none of these
The most widely used chemical for protoplast fusion, as fusogen is
Manitol
Sorbitol
Mannol
Polyethylene glycol
The first transgenic crop was
Pea
Tobacco
Flax
Cotton
The enzymes required to obtain wall-free / naked protoplasts are
Cellulase and Proteinase
Cellulase and Pectinase
Cellulase and amylase
Amylase and Pectinase
The ability of the component cells of callus to form a whole plant is known as
Redifferentiation
Dedifferentiation
either a or b
none of these
Synthetic seed is produced by encapsulating somatic embryo with
Sodium Chloride
Sodium alginate
Sodium acetate
Sodium nitrate
Subculturing is similar to propagation by cuttings because
it separates multiple microshoots and places them in a medium
it uses scions to produce new microshoots
they both use in vitro growing conditions
all of the abov
Somatic hybridization is achieved through
Grafting
Protoplast fusion
Conjugation
Recombinant DNA technology
Somaclonal variations are the ones
Caused by mutagens
Produce during tissue culture
Caused by gamma rays
Induced during sexual embryogeny
Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with
cellulotyic enzymes
pectolytic enzymes
both cellulotyic and pectolytic enzymes
proteolytic enzymes
Protoplast are the cells devoid of
cell membrane
cell wall
both cell wall and cell membrane
none of these
Polyethylene glycol is
Fusogenic chemical
Electrofusion stimulant
Callus stimulant
Differentiation stimulant
Plant tissue culture technique is a redefined method of
Vegetative Propagation
Asexual Reproduction
Selection
Hybridization
Part of plant used for culturing is called
Explant
Stock
Callus
Scion
Organogenesis is
formation of root and shoots on callus tissue
Both a and b
genesis of organs
formation of callus tissue
Media room of a plant tissue culture lab should consist of the following EXCEPT?
Autoclave machine
Analytical balance
Biosafety cabinet
pH meter
In plant tissue culture, what is the term ORGANOGENESIS means?
Formation of root & shoot from callus culture
Genesis of organ
None of the above
Formation of callus culture
In growth room, humidifier serves as?
Humidity reducer
Medium drying preventer
Temperature controller
Contaminant reducer