21.
In a cell, protoplast consists of the following EXCEPT?
A
Cell membrane
B
Nucleus
C
Cytoplasm
D
Cell wall
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In a cell, protoplast consists of the following EXCEPT?
Cell membrane
Nucleus
Cytoplasm
Cell wall
In a callus culture
increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
auxins and cytokinins are not required
only auxin is required for root and shoot formation
increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
Hormone pair required for a callus to differentiate are
Auxin and ethylene
Auxin and absiccic acid
Cytokinins and gibberllin
Auxin and cytokinin
Growth hormone producing apical dominance is
Gibberellin
Ethylene
Cytokinin
Auxin
DMSO (dimethyl sulfoxide) is used as
Alkylating agent
Chelating agent
Cryoprotectant
Gelling agent
Cybrids are produced by
Fusion of two same nuclei from same species
Nucleus of one species but cytoplasm from both the parent species
None of the above
Fusion of two different nuclei from two different species
Cellular totipotency is the property of
Animals
Bacteria
all of these
Plants
Cell fusion method includes the preparation of large number of
single plant cell stripped of their cell wall
plant cells with cell wall
cells from different species
plant cells stripped of their cell wall
Callus is
An insoluble carbohydrates
Tissue that grows to form embryoid
Un organized actively dividing mass of cells maintained in cultured
Tissue that forms embryo
Agrobacterium based gene transfer is efficient
only with dicots
only with monocots
with both monocots and dicots
with majority monocots and few dicots
A(n)...................is an excised piece of leaf or stem tissue used in micropropagation
microshoot
medium
explant
scion
A medium which is composed of chemically defined compound is called
Natural media
Synthetic media
Artificial media
None of these
Who is the father of tissue culture?
Bonner
Haberlandt
Laibach
Gautheret
Which vector is mostly used in crop improvement?
Plasmid
Cosmid
Phasmid
Agrobacterium
Which of the following plant cell will show totipotency?
Xylem vessels
Sieve tube
Meristem
Cork cells
Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?
Nurse medium
Nurse or feeder culture
Both a and b
None of these
Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?
Multiplication of sexually derived sterile hybrids
Less multiplication of disease free plants
Storage and transportation of propagates
Both b and c
Which method of plant propagation involves the use of girdling?
Grafting
Cuttings
Layering
Micropropagation
Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species?
Mass selection
Protoplast fusion
Transformation
Transpiration
When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?
2-5 days
5-10 days
10-15 days
15-17 days